Recombinant Human TNF beta/TNFB Protein

• Background:

  Tumor Necrosis Factor β (TNF-β) is a secreted protein belonging to the tumor necrosis factor family. TNF-β binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM in homotrimeric form, binds to TNFRSF3/LTBR in heterotrimeric form with LTB. TNF-β forms heterotrimers with lymphotoxin-beta, which anchors TNF-β to the cell surface. TNF-β mediates the inflammatory, immunostimulatory, and antiviral response, involves in the formation of second lymphoid organs during development, has a role in apoptosis. TNF-β is produced by lymphocytes and cytotoxic for a variety of tumor cells in vitro and in vivo.

• Abbreviation:

  TNF beta, TNFB

• Synonyms:

  LT-Alpha; LTA; Lymphotoxin-Alpha; TNF-Beta; TNFB; TNFSF1; Tumor Necrosis Factor Ligand Superfamily Member 1

• UniProt:

  P01374

• Accession Number:

  P01374

• Expression System:

  E. coli

• Tag:

  N-His

• Sequence:

  Leu35-Leu205

• Applications:

  Cell culture

• Field of Research:

  Signal Transduction; Cancer; immunology;

• Endotoxin:

  < 0.1 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 98 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED₅₀ for this effect is <3 pg/mL. The specific activity of recombinant human TNF beta is > 3.3x 10⁸ IU/mg.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  18.7 kDa

• Observed Molecular Weight:

  21 kDa

• Formulation:

  Lyophilized from sterile 20 mM sodium citrate, 0.2 M NaCl, pH 3.5.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human