Recombinant Human TFIIB/GTF2B Protein (GST Tag)

• Background:

  Transcription Initiation Factor IIB (TFIIB) is an essential factor for transcription by RNA Polymerase II. TFIIB localizes to the nucleus where it forms a complex (the DAB complex) with transcription factor IID and IIA. TFIIB plays a role as a bridge between IID, which initially recognizes the promoter sequence, and RNA polymerase II. TFIIB is involved in the selection of transcription start site.

• Abbreviation:

  TFIIB; GTF2B

• Synonyms:

  GTF2B; General Transcription Factor TFIIB; S300-II; TF2B; TFIIB; Transcription Initiation Factor IIB

• UniProt:

  Q00403

• Accession Number:

  Q00403

• Expression System:

  E.coli

• Tag:

  N-GST

• Sequence:

  Met 1-Leu316

• Field of Research:

  Epigenetics and nuclear signal

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 80 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  61.6 kDa

• Observed Molecular Weight:

  62 kDa

• Species:

  Human