Recombinant Human GRB2 Protein (His Tag)

• Background:

  As an adaptor protein, Growth Factor Receptor-Bound Protein 2 (GRB2) is involved in siganl transduction and consists of a central SH2 domain flanked by two SH3 domains. GRB2 associates with activated Tyr-phosphorylated EGF receptor/EGFR and PDGF receptors via its SH2 domain, stimulating GTP binding to Ras, which in turn activates MAPK and other signaling pathway.It also associates to other cellular Tyr-phosphorylated proteins such as SIT1, IRS1, IRS4, SHC and LNK. probably via the concerted action of both its SH2 and SH3 domains.

• Abbreviation:

  GRB2

• Synonyms:

  ASH; Adapter Protein GRB2; GRB2; Growth Factor Receptor-Bound Protein 2; Protein Ash; SH2/SH3 Adapter GRB2

• UniProt:

  P62993

• Accession Number:

  P62993

• Expression System:

  E.coli

• Tag:

  C-His

• Sequence:

  Met 1-Val217

• Field of Research:

  Signal Transduction; Cancer

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 90 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  26.3 kDa

• Observed Molecular Weight:

  25-30 kDa

• Species:

  Human