Recombinant Human Fumarase/FH Protein

• Background:

  Fumarase is an enzyme that catalyze the reversible hydration/dehydration of fumarate to S-malate and is involved in the tricarboxylic acid or Krebs cycle. Fumarase exists in both form, cytosolic formand N-terminal extend mitochondrial form. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension is the same form as in the cytoplasm. Fumarase is thought to act as a tumor suppressor, which deficiency can lead to progressive encephalopathy, cerebral atrophy and development delay.

• Abbreviation:

  Fumarase; FH

• Synonyms:

  FH; FMRD; Fumarase; Fumarate Hydratase Mitochondrial; HLRCC; LRCC; MCL; MCUL1

• UniProt:

  P07954

• Accession Number:

  P07954

• Expression System:

  E.coli

• Tag:

  None

• Sequence:

  Ala45-Lys510

• Field of Research:

  Signal Transduction; Cancer; metabolism

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  50.2 kDa

• Observed Molecular Weight:

  45 kDa

• Formulation:

  Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, pH 8.0.

• Species:

  Human