Recombinant Human CXCL7/NAP-2 Protein

• Background:

  Human Chemokine (C-X-C motif) Ligand 7 (CXCL7) ; also known as neutrophil activating peptide 2 (NAP-2) ; is a member of the CXC chemokines containing an ELR domain (Glu-Leu-Arg tripeptide motif) . Similar to other ELR domain containing CXC chemokines; such as IL-8 and the GRO proteins; CXCL7 binds CXCR2; chemoattracts and activates neutrophils. CXCL7; Connective Tissue Activating Protein III (CTAPIII) and βthrombogulin (βTG) ; are proteolytically processed carboxylterminal fragments of platelet basic protein (PBP) which is found in the alphagranules of human platelets. Although CTAPIII; βTG; and PBP represent amino-terminal extended variants of NAP2 and possess the same CXC chemokine domains; these proteins do not exhibit CXCL7/NAP2 activity. CXCL7 induces cell migration through the G-protein-linked receptor CXCR-2.

• Abbreviation:

  CXCL7; NAP-2

• Synonyms:

  C-X-C Motif Chemokine 7; CTAP3; CXCL7; LDGF; Leukocyte-Derived Growth Factor; MDGFSmall-Inducible Cytokine B7; Macrophage-Derived Growth Factor; PBP; PPBP; Platelet Basic Protein; SCYB7; TGB1; THBGB1

• UniProt:

  P02775

• Accession Number:

  P02775

• Expression System:

  E.coli

• Tag:

  N-His

• Sequence:

  Ala59-Asp128

• Applications:

  Cell culture

• Field of Research:

  Signal Transduction; Cardiovascular; immunology

• Endotoxin:

  < 0.1 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 98 % as determined by reducing SDS-PAGE.

• Bioactivity:

  50

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  8.4 kDa

• Observed Molecular Weight:

  11 kDa

• Species:

  Human