Recombinant Human ATG5 Protein

• Background:

  ATG5 is an E2 ubiquitin ligase which is necessary for autophagy. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity, dramatically highly expressed in apoptotic cells. It is activated by ATG7, conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate complex detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 plays an important role in the apoptotic process, possibly within the modified cytoskeleton.

• Abbreviation:

  ATG5

• Synonyms:

  APG5-like; APG5L; ASP; ATG5; Apoptosis-specific protein; Autophagy protein 5

• UniProt:

  Q9H1Y0

• Accession Number:

  Q9H1Y0

• Expression System:

  E.coli

• Tag:

  None

• Sequence:

  Met 1-Asp275

• Field of Research:

  Signal Transduction; Cell biology; Cardiovascular; Cancer; metabolism

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 90 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  32.4 kDa

• Observed Molecular Weight:

  33 kDa

• Formulation:

  Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 0.2M NaCl, 1mM DTT, 40% Glycerol, pH 8.0.

• Species:

  Human