Recombinant Human IL-1RAcP/IL-1R3 Protein (His Tag)

• Background:

  Interleukin-1 receptor accessory protein (IL-1RAcP) also known as Interleukin-1 receptor member 3 (IL-1R3) is a a cytokine receptor which binds interleukin 1. The IL-1 receptor accessory protein (IL1RAP) is a transmembrane protein that interacts with IL-1R and is required for IL-1 signal transduction. Interleukin 1 induces synthesis of acute phase and proinflammatory proteins during infection, tissue damage, or stress, by forming a complex at the cell membrane with an interleukin 1 receptor and an accessory protein. IL-1RAcP/IL-1R3 is a necessary part of the interleukin 1 receptor complex which initiates signalling events that result in the activation of interleukin 1-responsive genes. Alternative splicing of this gene results in two transcript variants encoding two different isoforms, one membrane-bound and one soluble. The ratio of soluble to membrane-bound forms increases during acute-phase induction or stress. IL-1RAcP/IL-1R3 mediates interleukin-1-dependent activation of NF-kappa-B. Isoform 1 is part of the membrane-bound form of the IL-1 receptor. Signaling involves formation of a ternary complex containing IL1R1, TOLLIP, MYD88, and IRAK1 or IRAK2. Isoform 2 modulates the response to interleukins by associating with soluble IL1R1 and enhancing interleukin-binding to the decoy receptor.

• Abbreviation:

  IL-1RAcP/IL-1R3

• Synonyms:

  IL1R; Interleukin-1 Receptor; C3orf; IL-1 Receptor Accessory Protein; IL-1R3; IL-1R-3; IL-1RAcP; Interleukin-1 Receptor 3; Interleukin-1 Receptor Accessory Protein; C3orf13; IL1R3; IL1RAP; FLJ37788; IL 1 receptor accessory protein; IL 1R3; IL 1RAcP; IL 1RAP; Interleukin 1 Accessory Protein; interleukin 1 receptor 3; Interleukin 1 receptor accessory protein

• UniProt:

  Q9NPH3

• Accession Number:

  NP_002173.1

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Met 1-Glu 359

• Field of Research:

  Cardiovascular; immunology

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 98 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  40.7 kDa

• Observed Molecular Weight:

  50-55 kDa

• Species:

  Human