Recombinant Human G-CSFR/CD114 Protein

• Background:

  Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as CD114, which belongs to the cytokine receptor superfamily, is a cell surface receptor for colony stimulating factor 3 (CSF3). It is a critical regulator of granulopoiesis. This type I membrane protein has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type III (FNIII) domains in the extracellular region. Mutations in the G-CSF receptor leading to carboxy-terminal truncation transduce hyperproliferative growth responses, and are implicated in the pathological progression of severe congenital neutropenia (SCN) to acute myelogenous leukemia (AML). Additionally, autocrine/paracrine stimulation of G-CSFR may be important in the biology of solid tumors, including metastasis.

• Synonyms:

  CD114, CSF3R, G-CSF R, GCSFR

• UniProt:

  Q99062

• Accession Number:

  NP_000751.1

• Host:

  HEK293 Cells

• Origin Species:

  Human

• Tag:

  None

• Sequence:

  Met 1-Pro 621

• Applications:

  Cell culture

• Field of Research:

  Cancer;immunology;Stem cells

• Purity:

  > 85 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured by its ability to inhibit GCSF-induced proliferation of NFS60 mouse myeloid cells. The ED50 for this effect is typically 2-8 μg/mL.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Molecular Weight:

  94 kDa

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• CAS Number:

  9000-83-3