Recombinant Human MERTK/Mer Protein

• Background:

  Proto-oncogene tyrosine-protein kinase MER (MERTK) is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains; two Ig-like C2-type (immunoglobulin-like) domains; and one tyrosine kinase domain. MERTK is localized in membrane and is no expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. This protein is highly expressed in testis; ovary; prostate; lung; and kidney; with lower expression in spleen; small intestine; colon; and liver. MERTK regulates many physiological processes including cell survival; migration; differentiation; and phagocytosis of apoptotic cells (efferocytosis) . Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells; platelet aggregation; cytoskeleton reorganization and engulfment. MERTK plays also an important role in inhibition of Toll-like receptors (TLRs) -mediated innate immune response by activating STAT1; which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. Defects in MERTK are the cause of retinitis pigmentosa type 38.

• Synonyms:

  MER; MERTK; Mer; Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK; Tyrosine-protein kinase Mer

• UniProt:

  Q12866

• Accession Number:

  NP_006334.2

• Expression System:

  HEK293 Cells

• Tag:

  None

• Sequence:

  Met 1-Ala 499

• Field of Research:

  Signal Transduction; Cell biology; Neuroscience; immunology; metabolism

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 85 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  54.0 kDa

• Observed Molecular Weight:

  110-120 kDa

• Species:

  Human