Recombinant Human PARP-1 Protein (His Tag)

Recombinant Human PARP-1 Protein (His Tag)

• Background: Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrateed to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors is thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.
• Synonyms: ADPRT, ADPRT1, ARTD1, PARP, PARP-1, PPOL, pADPRT-1
• CAS Number: 9000-83-3
• UniProt: P09874
• Accession Number: NP_001609.2
• Host: Baculovirus
• Origin Species: Human
• Tag: C-His
• Sequence: Met 1-Trp 1014
• Applications: ELISA
• Field of Research: Cell biology;Cancer;epigenetics and nuclear signal;metabolism
• Purity: > 90 % as determined by reducing SDS-PAGE.
• Bioactivity: Immobilized human PARP1 at 10 μg/mL (100 μl/well) can bind biotinylated human HSP70, The EC50 of biotinylated human HSP70 is 0.035 μg/mL.
• Reconstitution: Not Applicable
• Molecular Weight: 100-110 kDa
• Shipping Conditions: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < -20°C.
• Storage Conditions: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.