Recombinant Human SRC Kinase/c-SRC Protein (His & GST Tag)

• Background:

  Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is a constitutively active as opposed to normal SRC (C-SRC) . Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity.

• Synonyms:

  ASV; SRC1; c-SRC; p60-Src

• UniProt:

  P12931

• Accession Number:

  P12931-1

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  N-His-GST

• Sequence:

  Met 1-Leu 536

• Applications:

  Enzyme

• Field of Research:

  Signal Transduction, Epigenetics and Nuclear Signaling, Cancer

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 90 % as determined by reducing SDS-PAGE.

• Bioactivity:

  The specific activity was determined to be > 80 nmol/min/mg using Poly (Glu:Tyr) 4:1 as substrate.

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  87.7 kDa

• Observed Molecular Weight:

  81 kDa

• Formulation:

  Supplied as sterile solution of 50mM Tris, 100mM NaCl, pH 8.0, 20% glycerol, 0.3mM DTT

• Species:

  Human