EZH2 Polyclonal Antibody

• Background:

  EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa) . (20935635,21367748)

• Abbreviation:

  EZH2

• UniProt:

  Q15910, Q61188

• Host:

  Rabbit

• Reactivity:

  Human; Mouse

• Immunogen:

  KLH conjugated Synthetic peptide corresponding to Mouse EZH2

• Target:

  Enhancer of zeste 2; enhancer of zeste 2 polycomb repressive complex 2 subunit; Enhancer of zeste homolog 2 (Drosophila) ; Enhancer of zeste homolog 2; Enhancer of zeste; Drosophila; homolog 2; ENX 1; Enx 1h; ENX-1; ENX1; Enx1h; EZH 2; EZH1; EZH2; EZH2; EZH2b; Histone-lysine N-methyltransferase EZH2; KMT 6; KMT6; KMT6A; Lysine N-methyltransferase 6; MGC9169; WVS; WVS2

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Applications:

  WB

• Field of Research:

  Cancer; Epigenetics and Nuclear Signaling

• Purification:

  Affinity purification

• Concentration:

  0.54 mg/mL

• Dilution:

  WB 1:500-1:2000

• Buffer:

  PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4

• Shipping Conditions:

  The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  80-100kDa

• Observed Molecular Weight:

  80kDa

• Isotype:

  IgG