PRKDC Polyclonal Antibody

• Background:

  Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V (D) J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V (D) J recombination by activation of the hairpin endonuclease artemis (DCLRE1C) . The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.

• Abbreviation:

  DNA-PKCS

• UniProt:

  P78527

• Host:

  Rabbit

• Reactivity:

  Human; Mouse

• Immunogen:

  Synthesized peptide derived from the C-terminal region of human DNA-PKCS

• Target:

  DNA dependent protein kinase catalytic subunit; DNA PK catalytic subunit; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNAPK; DNAPK catalytic subunit; DNPK 1; DNPK1; Hyper radiosensitivity of murine scid mutation; complementing 1; Hyperradiosensitivity complementing 1; mouse; homolog of 1; HYRC 1; HYRC; HYRC1; IMD26; p350; p460; PKRDC; PRKDC; PRKDC; Protein Kinase DNA Activated Catalytic Polypeptide; XRCC 7; XRCC7

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Applications:

  WB; IHC-p; IF

• Field of Research:

  Cancer; Epigenetics and Nuclear Signaling

• Purification:

  Affinity purification

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000

• Buffer:

  PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

• Shipping Conditions:

  The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  469kDa

• Observed Molecular Weight:

  450kDa

• Isotype:

  IgG