Purified Rat IgG1, κ Isotype Control[HRPN]

• Background:

  CD272, also known as B and T lymphocyte attenuator (BTLA), is an Ig superfamily co-inhitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4. BTLA is expressed on B cells, T cells, macrophages, dendritic cells, NKT cells, and NK cells. Engagement of BTLA by its ligand herpes virus entry mediator (HVEM) is critical for negatively regulating immune response. The absence of BTLA with HVEM inhibitory interactions leads to increased experimental autoimmune encephalomyelitis severity, enhanced rejection of partially mismatched allografts, an increased CD8+ memory T cell population, increased severity of colitis, and reduced effectiveness of T regulatory cells. BTLA plays an important role in the induction of peripheral tolerance of both CD4+ and CD8+ T cells in vivo. Tolerant T cells have significantly higher expression of BTLA compared with effectors and naïve T cells. BTLA may cooperate with CTLA-4 and PD-1 to control T cell tolerance and autoimmunity. It was reported that BTLA may regulate T cell function by binding to B7-H4, but further studies are needed to confirm. The existence of three distinct BTLA alleles has been reported.

• Host:

  Rat

• Clonality:

  Monoclonal

• Clone:

  HRPN

• Conjugation:

  Unconjugated

• Applications:

  FCM

• Concentration:

  ≥ 1 mg/mL

• Buffer:

  Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.

• Recommended Usage:

  Each lot of this antibody is quality control tested by flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.

• Isotype:

  Rat IgG1, κ