Pepsin Activity Assay Kit

• Type:

  Colorimetric method

• Field of Research:

  Amino Acids and Proteins

• Assay Principle:

  Pepsin can catalyze substrate hydrolysis, and the hydrolysate reacts with the chromogenic agent under alkaline conditions to form a blue substance. The blue substance has a maximum absorption peak at 660 nm, and the color depth is proportional to the activity of pepsin. The enzyme activity can be calculated by OD value

• Sample Type:

  Gastric; intestinal tissue

• Detection Range:

  0.63-94.98 U/mL

• Sensitivity:

  0.63 U/mL

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Inter CV:

  4.8-5.6%

• Intra CV:

  1.3-2.2%

• Detection Instrument:

  Microplate reader