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Sucrase Activity Assay Kit

CAT: 0763-E-BC-K751-MSize: 96 T (40 samples)Dry Ice: NoHazardous: No
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CAT#:0763-E-BC-K751-MSize:96 T (40 samples)
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Type
Colorimetric method
Field of Research
Inorganic Ions; Plant Physiology Series
Assay Principle
Sucrase catalyzes its substrate (sucrose) to produce glucose, which produces hydrogen peroxide under the action of glucose oxidase. Hydrogen peroxide reacts with chromogenic agent to produce red substance, which has a strong absorption peak at 505 nm. In a certain concentration range, It's absorbance is proportional to glucose concentration. Therefore, the activity of sucrase can be calculated by measuring the OD value at 505 nm.
Assay Performance Time
70 min
Sample Type
Animal tissu
Detection Range
20-2000 U/mL
Sensitivity
20 U/mL
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at 2~8°C for 12 months with shading light.
Inter CV
6.5%
Intra CV
5.4%
Detection Instrument
Microplate reader
Product Datasheet
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