Sucrase Activity Assay Kit

• Type:

  Colorimetric method

• Field of Research:

  Inorganic Ions; Plant Physiology Series

• Assay Principle:

  Sucrase catalyzes its substrate (sucrose) to produce glucose, which produces hydrogen peroxide under the action of glucose oxidase. Hydrogen peroxide reacts with chromogenic agent to produce red substance, which has a strong absorption peak at 505 nm. In a certain concentration range, It's absorbance is proportional to glucose concentration. Therefore, the activity of sucrase can be calculated by measuring the OD value at 505 nm.

• Assay Performance Time:

  70 min

• Sample Type:

  Animal tissu

• Detection Range:

  20-2000 U/mL

• Sensitivity:

  20 U/mL

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at 2~8°C for 12 months with shading light.

• Inter CV:

  6.5%

• Intra CV:

  5.4%

• Detection Instrument:

  Microplate reader