NAD-Isocitrate Dehydrogenase (NAD-IDH) Activity Assay Kit

CAT:

763-E-BC-K651-M

Size:

96 Tests

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Synonyms: NAD-IDH
Field of Research: Tricarboxylic Acid（TCA）Cycle
Detection Method: Colorimetric method
Assay Principle: Isocitrate dehydrogenase (IDH) is one of the inverters in the tricarboxylic acid cycle and plays an important role in energy metabolism, amino acid and vitamin synthesis. The cofactors of this enzyme include two kinds of NAD+ and NADP+, which are located in different parts of the cell respectively. In eukaryotic cells, NAD-dependent isocitrate dehydrogenase mainly exists in mitochondria. Under the activation of the activator, IDH converts isocitrate into α-ketoglutaric acid. Meanwhile, NAD+ is reduced to NADH, which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of NAD-IDH can be calculated by measuring the change of absorbance value at 450 nm.
Assay Performance Time: 50 min
Detection Range: 0.84-50 U/L
Sensitivity: 0.84 U/L
Shipping Conditions: Ice bag
Storage Conditions: This product can be stored at -20°C for 12 months with shading light.

Cat	Name
E-BC-K651-M	NAD-Isocitrate Dehydrogenase (NAD-IDH) Activity Assay Kit
E-BC-K554-M	NADP-Isocitrate Dehydrogenase (NADP-IDH) Activity Assay Kit
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PA7185-02	IDH-1 Monoclonal Antibody
PA7185-03	IDH-1 Monoclonal Antibody
ELAB6945-R-03	IDH-1 Monoclonal Antibody

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