NADP-Isocitrate Dehydrogenase (NADP-IDH) Activity Assay Kit

• Type:

  Colorimetric method

• Field of Research:

  Glycolysis and Lipid Metabolism; Tricarboxylic Acid (TCA) Cycle

• Assay Principle:

  Isocitrate dehydrogenase converts isocitrate to α- ketoglutaric acid, while converting NADP+ to NADPH. Under the action of electron coupling agents, the generated NADPH has a characteristic absorption peak at 450 nm. The activity of NADP-IDH can be calculated by measuring the change of absorbance value at 450 nm.

• Assay Performance Time:

  70 min

• Sample Type:

  Tissue; cell

• Detection Range:

  0.10-50.47 U/L

• Sensitivity:

  0.10 U/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Inter CV:

  4.3-11.4%

• Intra CV:

  5.0-6.0%

• Detection Instrument:

  Microplate reader