Creatinine (Cr) Colorimetric Assay Kit (Sarcosine Oxidase Method)

• Target:

  Cr

• Type:

  Colorimetric method

• Field of Research:

  Liver and Renal Function

• Assay Principle:

  Creatinine (Cr) can be catalyzed by creatinase and generates creatine. Creatine can be hydrolyzed into sarcosine and urea by creatinase. The sarcosine can be catalyzed by sarcosine oxidase and form glycine, formaldehyde and hydrogen peroxide. The reaction between hydrogen peroxide, 2,4- (6-Tri-iodine-3- hydroxybenzoic acid) and 4-ampyrone can be catalyzed by peroxidase and form pink compound. Creatinine content can be calculated indirectly by measuring the OD value at 515 nm.

• Assay Performance Time:

  30 min

• Sample Type:

  Serum; plasma; urine

• Detection Range:

  20.45-400 μmol/L

• Sensitivity:

  3.8 μmol/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at 2~8°C for 12 months with shading light.

• Citation 01:

  https://www.sciencedirect.com/science/article/pii/S0142961221003434

• Citation 02:

  https://www.sciencedirect.com/science/article/pii/S1742706121007108

• Citation 03:

  https://www.sciencedirect.com/science/article/pii/S1742706120307509

• Citation 04:

  https://pubs.rsc.org/en/content/articlelanding/2023/bm/d2bm01836e

• Citation 05:

  https://www.hindawi.com/journals/omcl/2022/1763922/

• Citation 06:

  https://www.mdpi.com/2124124

• Citation 07:

  https://link.springer.com/article/10.1007/s10565-023-09790-0

• Citation 08:

  https://www.mdpi.com/article/10.3390/ijms232315400

• Citation 09:

  https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.7608

• Citation 10:

  https://europepmc.org/article/med/35392552?javascript_support=no

• Citation 11:

  https://www.sciencedirect.com/science/article/pii/S0378874123001113

• Citation 12:

  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164873/

• Inter CV:

  3.7%

• Intra CV:

  1.4%

• Detection Instrument:

  Microplate reader