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Cr (Creatinine) ELISA Kit

CAT: 0763-E-EL-0058-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-0058-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
Cr
Cross Reactivity
This kit recognizes Universal Cr in samples.No significant cross-reactivity or interference between Universal Cr and analogues was observed
Target
2-Amino-1-methyl-5H-imidazol-4-one;2-Amino-1-methylimidazol-4-ol
Type
Competitive-ELISA
Applications
ELISA
Field of Research
Cancer; Neuroscience
Assay Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal Cr. During the reaction, Universal Cr in the sample or standard competes with a fixed amount of Universal Cr on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal Cr. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal Cr in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Performance Time
2 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
1.25-80 μg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
0.75 μg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.sciencedirect.com/science/article/pii/S0753332222013361
Citation 02
https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/1751-7915.14200
Citation 03
https://www.mdpi.com/1436372
Citation 04
https://link.springer.com/article/10.1007/s12079-022-00685-z
Citation 05
https://www.mdpi.com/2218-1989/12/9/775
Citation 06
https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.202101030R
Citation 07
https://www.sciencedirect.com/science/article/pii/S0300483X22000270
Citation 08
https://pubmed.ncbi.nlm.nih.gov/33015817/
Citation 09
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0259132
Citation 10
https://www.sciencedirect.com/science/article/pii/S0211699521000576
Citation 11
https://link.springer.com/article/10.1007/s11255-020-02485-z
Citation 12
https://www.spandidos-publications.com/mmr/20/4/3337
Detection Instrument
Microplate reader
Species
Universal
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Cr concentrations in serum, plasma and other biological fluids.

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