Mitochondrial Complex V (F0F1-ATPase/ATP Synthase) Activity Assay Kit

• Target:

  ATP Synthase

• Type:

  Colorimetric method

• Field of Research:

  Mitochondria Function; cuproptosis

• Assay Principle:

  Mitochondrial complex V is also known as F0F1-ATP synthase. ATP is hydrolyzed by F0F1-ATP synthase to produce ADP, and ADP converts NADH into oxidized coenzyme I (NAD) after enzyme conversion reaction. Therefore, the activity of mitochondrial complex IV can be quantified by measure the change OD value at 340 nm.

• Assay Performance Time:

  15 min

• Sample Type:

  Animal tissue; cell

• Detection Range:

  12.13-74.21 U/L

• Sensitivity:

  12.13 U/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Inter CV:

  6.5%

• Intra CV:

  5.6%

• Detection Instrument:

  Microplate reader