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Ethanol Colorimetric Assay Kit

CAT: 0763-E-BC-K891-M-01Size: 48 T (32 samples)Dry Ice: NoHazardous: No
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CAT#:0763-E-BC-K891-M-01Size:48 T (32 samples)
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Type
Colorimetric method
Field of Research
Liver and Renal Function
Assay Principle
Ethanol dehydrogenase can catalyze oxidative dehydrogenation of ethanol to acetaldehyde, and NAD+ is reduced to produce NADH. NADH, under the action of 1-mPMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, ethanol content can be quantified by measure the OD value at 450 nm.
Assay Performance Time
30 min
Sample Type
Serum; plasma; wine
Detection Range
0.27–10.0 μmol/mL
Sensitivity
0.27 μmol/mL
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Inter CV
5%
Intra CV
3.5%
Detection Instrument
Microplate reader

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