Ethanol Colorimetric Assay Kit

• Type:

  Colorimetric method

• Field of Research:

  Liver and Renal Function

• Assay Principle:

  Ethanol dehydrogenase can catalyze oxidative dehydrogenation of ethanol to acetaldehyde, and NAD+ is reduced to produce NADH. NADH, under the action of 1-mPMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, ethanol content can be quantified by measure the OD value at 450 nm.

• Assay Performance Time:

  30 min

• Sample Type:

  Serum; plasma; wine

• Detection Range:

  0.27–10.0 μmol/mL

• Sensitivity:

  0.27 μmol/mL

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Inter CV:

  5%

• Intra CV:

  3.5%

• Detection Instrument:

  Microplate reader