Total Glutathione (T-GSH) /Oxidized Glutathione (GSSG) Colorimetric Assay Kit

• Target:

  T-GSH; GSSG

• Type:

  Colorimetric method

• Field of Research:

  Oxidative stress

• Assay Principle:

  GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with appropriate reagent and then using the above reaction principle.

• Assay Performance Time:

  180 min

• Sample Type:

  Serum; plasma; animal tissue; red blood cells; cultured cells

• Detection Range:

  0.12-30 μmol/L T-GSH

• Sensitivity:

  0.12 μmol/L T-GSH

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.

• Citation 01:

  https://www.sciencedirect.com/science/article/pii/S0308814621004301

• Citation 02:

  https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo03577k

• Citation 03:

  https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.15397

• Citation 04:

  https://www.tandfonline.com/doi/abs/10.1080/13880209.2022.2070221

• Citation 05:

  https://porcinehealthmanagement.biomedcentral.com/articles/10.1186/s40813-022-00275-w

• Citation 06:

  https://onlinelibrary.wiley.com/doi/abs/10.1002/jsfa.11611

• Citation 07:

  https://www.tandfonline.com/doi/abs/10.1080/21655979.2021.2024319

• Inter CV:

  4.7%

• Intra CV:

  0.9%

• Detection Instrument:

  Spectrophotometer