Glutathione Peroxidase (GSH-Px) Activity Assay Kit

• Target:

  GSH-PX

• Type:

  Colorimetric method

• Field of Research:

  Oxidative stress; Plant Physiology Series; Ferroptosis

• Assay Principle:

  Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG) . The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH.

• Assay Performance Time:

  40 min

• Sample Type:

  Serum; plasma; cells; cell culture supernatant; tissue

• Detection Range:

  34.34-1036.64 U

• Sensitivity:

  34.34 U

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at 2~8°C for 12 months with shading light.

• Citation 01:

  https://www.sciencedirect.com/science/article/pii/S0891584922009777

• Citation 02:

  https://www.mdpi.com/1905716

• Citation 03:

  https://pubmed.ncbi.nlm.nih.gov/36330218/

• Citation 04:

  https://www.sciencedirect.com/science/article/pii/S0278691521001575

• Citation 05:

  https://www.sciencedirect.com/science/article/pii/S0278691522002599

• Citation 06:

  https://www.sciencedirect.com/science/article/pii/S0753332221003553

• Citation 07:

  https://www.sciencedirect.com/science/article/pii/S0753332221008003

• Citation 08:

  https://www.mdpi.com/1926090

• Citation 09:

  https://www.mdpi.com/2072-6643/14/15/3179

• Citation 10:

  https://www.mdpi.com/2076-3921/9/3/250

• Citation 11:

  https://www.mdpi.com/1332228

• Citation 12:

  https://europepmc.org/article/pmc/pmc9500298?javascript_support=no

• Inter CV:

  8.7%

• Intra CV:

  2.4%

• Detection Instrument:

  Microplate reader