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Hydroxyproline (HYP) Colorimetric Assay Kit (Acid Hydrolysis Method)

CAT: 0763-E-BC-K062-M-01Size: 48 T (32 samples)Dry Ice: NoHazardous: No
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CAT#:0763-E-BC-K062-M-01Size:48 T (32 samples)
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Target
HYP
Type
Colorimetric method
Field of Research
Amino Acids and Proteins
Assay Principle
The sample is hydrolyzed to generate free HYP, and hydroxyproline can produce oxidation product under the action of oxidizing agent. The generated oxidation product can react with chromogenic agent to produce burgundy. The concentration of hydroxyproline can be calculated by measuring the OD value at 558 nm.
Assay Performance Time
50 min
Sample Type
Serum; animal tissue; urine
Detection Range
0.04-10 μg/mL
Sensitivity
0.04 μg/mL
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at 2~8°C for 12 months with shading light.
Citation 01
https://www.sciencedirect.com/science/article/pii/S0091674922013422
Inter CV
4.4%
Intra CV
1.2%
Detection Instrument
Microplate reader
Product Datasheet
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