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QuicKey Pro Mouse E2 (Estradiol) ELISA Kit

CAT: 0763-E-OSEL-M0008-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-OSEL-M0008-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
E2
Cross Reactivity
This kit recognizes Mouse E2 in samples.No significant cross-reactivity or interference between Mouse E2 and analogues was observed
Target
Oestradiol; E2;17β-Estradiol; Estra-1;3;5 (10) -triene-3;17β-diol
Type
Competitive-ELISA
Applications
ELISA
Field of Research
Cancer; Cardiovascular; Metabolism; Neuroscience; Signaling transduction
Assay Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse E2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse E2 are added to the micro ELISA plate wells. Mouse E2 in samples (or standards) competes with a fixed amount of E2 on the solid phase supporter for sites on the HRP linked detection antibody specific to E2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Mouse E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
1 h 30 min
Sample Type
Serum, Plasma, Urine, Saliva
Detection Range
3.13-200 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
1.88 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,6 months
Citation 01
https://www.sciencedirect.com/science/article/pii/S0304389421017829
Citation 02
https://www.sciencedirect.com/science/article/pii/S0269749122016499
Citation 03
https://www.nature.com/articles/s41419-021-04277-4
Citation 04
https://www.sciencedirect.com/science/article/pii/S0269749121011398
Citation 05
https://www.sciencedirect.com/science/article/pii/S0160412020322534
Citation 06
https://www.sciencedirect.com/science/article/pii/S0147651322007679
Citation 07
https://www.tandfonline.com/doi/abs/10.1080/17435390.2018.1537411
Citation 08
https://www.sciencedirect.com/science/article/pii/S0269749119332531
Citation 09
https://www.ncbi.nlm.nih.gov/pmc/articles/pmc7993734/
Citation 10
https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo04239d
Citation 11
https://www.nature.com/articles/srep21776/
Citation 12
https://rbej.biomedcentral.com/articles/10.1186/s12958-021-00702-7
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse E2 concentrations in Serum, Plasma, Urine, Saliva.

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