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Mini Sample Mouse IFN-γ (Interferon Gamma) ELISA Kit

CAT: 0763-E-MSEL-M0007-01Size: 10x 96 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-MSEL-M0007-01Size:10x 96 Tests
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Abbreviation
IFN-γ
UniProt
P01580
Cross Reactivity
This kit recognizes Mouse IFN-γ in samples.No significant cross-reactivity or interference between Mouse IFN-γ and analogues was observed
Target
IFN-γ
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Immunology; Stem cells
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFN-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IFN-γ. You can calculate the concentration of Mouse IFN-γ in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
7.81-500 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
4.53 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.202208923
Citation 02
https://www.sciencedirect.com/science/article/pii/S1385894722059460
Citation 03
https://www.sciencedirect.com/science/article/pii/S1385894722060995
Citation 04
https://www.science.org/doi/abs/10.1126/scitranslmed.aaz6667
Citation 05
https://www.sciencedirect.com/science/article/pii/S0142961222005427
Citation 06
https://www.science.org/doi/abs/10.1126/sciadv.abg8335
Citation 07
https://www.sciencedirect.com/science/article/pii/S2211383523000643
Citation 08
https://jitc.bmj.com/content/10/1/e003809.abstract
Citation 09
https://jitc.bmj.com/content/10/6/e004381.abstract
Citation 10
https://link.springer.com/article/10.1186/s12951-022-01287-y
Citation 11
https://pubs.acs.org/doi/abs/10.1021/acsami.2c14448
Citation 12
https://pubs.acs.org/doi/abs/10.1021/acsami.2c04894
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse IFN-γ concentrations in serum, plasma and other biological fluids.

UniProtKB · P01580

Interferon gamma

IFNG_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P01580
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ifng
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
IFN-gamma
EC number
—
Processing
Precursor
Secondary accessions
Q542B8
Protein keywords

Biological process

Antiviral defenseGrowth regulation

Molecular function

Cytokine

PTM

Glycoprotein

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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