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Rat Irisin ELISA Kit

CAT: 0763-E-EL-R2625-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-R2625-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
Irisin
UniProt
Q8K3V5
Cross Reactivity
This kit recognizes Rat Irisin in samples.No significant cross-reactivity or interference between Rat Irisin and analogues was observed
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cell Biology; Metabolism
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Irisin. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Irisin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Irisin, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat Irisin. You can calculate the concentration of Rat Irisin in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
78.13-5000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
46.88 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.nature.com/articles/s12276-022-00811-2
Citation 02
https://www.sciencedirect.com/science/article/pii/S2589004223000676
Citation 03
https://www.hindawi.com/journals/omcl/2021/7382900/
Citation 04
https://www.sciencedirect.com/science/article/pii/S0188440919301274
Citation 05
https://link.springer.com/article/10.1007/s11332-022-00924-1
Species
Rat
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Rat Irisin concentrations in ..

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