Rat E-Cad (E-Cadherin) ELISA Kit

CAT: 0763-E-EL-R0347-01Size: 24 TestsDry Ice: NoHazardous: No

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CAT#:0763-E-EL-R0347-01Size:24 Tests

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Abbreviation

E-Cad

UniProt

Q9R0T4

Cross Reactivity

This kit recognizes Rat E-Cad in samples. No significant cross-reactivity or interference between Rat E-Cad and analogues was observed

Target

CDH1; Arc-1; CD324; CDHE; LCAM; UVO; CAM 120/80; Epithelial Cadherin; Uvomorulin

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Developmental biology; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat E-Cad. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat E-Cad and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat E-Cad, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat E-Cad. You can calculate the concentration of Rat E-Cad in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0024320522006993

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat E-Cad concentrations in serum, plasma and other biological fluids.

UniProtKB · Q9R0T4

Cadherin-1

CADH1_RAT · Rattus norvegicus

View on UniProt ↗

Primary accession: Q9R0T4
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: Cdh1
Protein existence: 1: Evidence at protein level
Organism: Rattus norvegicus (Rat)
Taxonomy ID: 10116
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: O35794, Q9JIV9

Protein keywords

Ligand

CalciumMetal-binding

Biological process

Cell adhesion

Cellular component

Cell junctionCell membraneCytoplasmEndosomeGolgi apparatusMembrane

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinPhosphoproteinUbl conjugation

Technical term

Reference proteome

Domain

RepeatSignalTransmembraneTransmembrane helix

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