Human E-Cad (E-Cadherin) ELISA Kit

CAT: 0763-E-EL-H0014-01Size: 24 TestsDry Ice: NoHazardous: No

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CAT#:0763-E-EL-H0014-01Size:24 Tests

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Abbreviation

E-Cad

UniProt

P12830

Cross Reactivity

This kit recognizes Human E-Cad in samples. No significant cross-reactivity or interference between Human E-Cad and analogues was observed

Target

CDH1; Arc-1; CD324; CDHE; LCAM; UVO; CAM 120/80; Epithelial Cadherin; Uvomorulin

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Developmental biology; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human E-Cad and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human E-Cad, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human E-Cad. You can calculate the concentration of Human E-Cad in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/2064064

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1111/jop.13388

Citation 03

https://link.springer.com/article/10.1007/s10157-019-01744-3

Citation 04

https://www.hindawi.com/journals/ecam/2019/7656105/abs/

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human E-Cad concentrations in serum, plasma and other biological fluids.

UniProtKB · P12830

Cadherin-1

CADH1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P12830
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: CDH1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: A8K1U7, Q13799, Q14216, Q15855, Q16194, Q4PJ14, Q9UII8

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

CalciumMetal-binding

Biological process

Cell adhesion

Cellular component

Cell junctionCell membraneCytoplasmEndosomeGolgi apparatusMembraneSecreted

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinPhosphoproteinUbl conjugation

Disease

Disease variantEctodermal dysplasia

Domain

RepeatSignalTransmembraneTransmembrane helix

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