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Human E-Cad (E-Cadherin) ELISA Kit

CAT: 0763-E-EL-H0014-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-H0014-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
E-Cad
UniProt
P12830
Cross Reactivity
This kit recognizes Human E-Cad in samples. No significant cross-reactivity or interference between Human E-Cad and analogues was observed
Target
CDH1; Arc-1; CD324; CDHE; LCAM; UVO; CAM 120/80; Epithelial Cadherin; Uvomorulin
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Developmental biology; Signal transduction
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human E-Cad and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human E-Cad, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human E-Cad. You can calculate the concentration of Human E-Cad in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.16-10 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
0.1 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.mdpi.com/2064064
Citation 02
https://onlinelibrary.wiley.com/doi/abs/10.1111/jop.13388
Citation 03
https://link.springer.com/article/10.1007/s10157-019-01744-3
Citation 04
https://www.hindawi.com/journals/ecam/2019/7656105/abs/
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human E-Cad concentrations in serum, plasma and other biological fluids.

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