Mouse IgE (Immunoglobulin E) ELISA Kit

CAT: 0763-E-EL-M3034-01Size: 24 TestsDry Ice: NoHazardous: No

1 / 1

CAT#:0763-E-EL-M3034-01Size:24 Tests

Selected

24/48H Stock Items & 2 to 6 Weeks non Stock Items.

Quick Request Actions

CAS Number

7732-18-5

Abbreviation

IgE

Synonyms

IgE

UniProt

F6W6U3

Species Reactivity

Mouse

Cross Reactivity

This kit recognizes Mouse IgE in samples.No significant cross-reactivity or interference between Mouse IgE and analogues was observed

Target

Mouse

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Immunology

Detection Method

Colormetric

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IgE. You can calculate the concentration of Mouse IgE in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

125-8000 ng/mL

Precision

Both intra-CV and inter-CV are < 10%

Recovery

80%-120%

Sensitivity

75 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C/-20°C,12 months

Applications Notes

This ELISA kit applies to the in vitro quantitative determination of Mouse IgE concentrations in serum, plasma and other biological fluids.

Citation 01

https://www.sciencedirect.com/science/article/pii/S0048969722041730

Chemical Information

Water

Water is an oxygen hydride consisting of an oxygen atom that is covalently bonded to two hydrogen atoms. It has a role as an amphiprotic solvent, a mouse metabolite, a Saccharomyces cerevisiae metabolite, a member of greenhouse gas, an Escherichia coli metabolite and a human metabolite. It is an oxygen hydride, a mononuclear parent hydride and an inorganic hydroxy compound. It is a conjugate base of an oxonium. It is a conjugate acid of a hydroxide.

CAS Number	7732-18-5
PubChem CID	962
IUPAC Name	oxidane
Molecular Formula	H2O
Molecular Weight	18.015
XLogP	-0.5
Topological Polar Surface Area	1
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	1
Rotatable Bond Count	0
Heavy Atom Count	1
Formal Charge	0
Complexity	0

SMILES

InChI

InChI=1S/H2O/h1H2

InChIKey

XLYOFNOQVPJJNP-UHFFFAOYSA-N

Chemical Structure

2D Structure

CAS: 7732-18-5

CID: 962

Formula: H2O

MW: 18.015

Interactive 3D Structure

Loading 3D structure...

View on PubChem Raw 3D data

Computed Properties

Property	Value
Molecular Weight	18.015
XLogP3	-0.5
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	1
Rotatable Bond Count	0
Exact Mass	18.010564683
Monoisotopic Mass	18.010564683
Topological Polar Surface Area	1 Å²
Heavy Atom Count	1
Formal Charge	0
Complexity	0
Isotope Atom Count	0
Defined Atom Stereocenter Count	0
Undefined Atom Stereocenter Count	0
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	0
Covalently-Bonded Unit Count	1
Compound Is Canonicalized	Yes

UniProtKB · F6W6U3

Immunoglobulin heavy constant epsilon

F6W6U3_MOUSE · Mus musculus

View on UniProt ↗

Primary accession: F6W6U3
Review status: UniProtKB unreviewed (TrEMBL)
Gene: Ighe
Protein existence: 1: Evidence at protein level
Organism: Mus musculus (Mouse)
Taxonomy ID: 10090
Alternative names: —
EC number: —
Processing: Fragment
Secondary accessions: —

Protein keywords

Cellular component

Cell membraneMembraneSecreted

PTM

Disulfide bond

Domain

Immunoglobulin domain

Technical term

Proteomics identificationReference proteome

Related Products

Catalog	Name
E-EL-M0691	ELISA kit for Mouse IgE (Immunoglobulin E)
E-EL-MK0021-01	Monkey IgE-Immunoglobulin E- ELISA Kit
E-EL-R0517-01	Rat IgE (Immunoglobulin E) ELISA Kit
E-EL-H6104-01	Human IgE (Immunoglobulin E) ELISA Kit
E-EL-H2161	ELISA kit for Human IgE (Immunoglobulin E)
E-EL-RB0659-01	Rabbit IgE-Immunoglobulin E- ELISA Kit
E-EL-P0286	ELISA kit for Porcine IgE (Immunoglobulin E)
E-CL-M0426-01	Mouse IgE (Immunoglobulin E) CLIA Kit
E-UNEL-H0037-01	Uncoated Human IgE (Immunoglobulin E) ELISA Kit
E-TSEL-H0016-01	QuicKey Human IgE-Immunoglobulin E- ELISA Kit

Alternative Products

Catalog	Name
MBS720755-01	Mouse Immunoglobulin A ELISA Kit
MBS3806488-01	Mouse Immunoglobulin ELISA Kit
JOT-EK0092Mo-01	Mouse Immunoglobulin,IgA ELISA KIT
EA0070Mo	Mouse Immunoglobulin A, IgA ELISA KIT
MBS725903-01	Mouse Immunoglobulin D ELISA Kit
MBS749281-01	Mouse Immunoglobulin G2 ELISA Kit
MBS2506544-01	Mouse IgA (Immunoglobulin A) ELISA Kit
MBS2018930-01	Mouse Immunoglobulin A (IgA) ELISA Kit
MBS032852-01	Mouse Immunoglobulin G ELISA Kit
MBS3806596-01	Mouse Immunoglobulin A Antibody ELISA Kit