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Mouse LTF/LF (Lactoferrin) ELISA Kit

CAT: 0763-E-EL-M0746-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-M0746-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
LTF; LF
UniProt
P08071
Cross Reactivity
This kit recognizes Mouse LTF/LF in samples. No significant cross-reactivity or interference between Mouse LTF/LF and analogues was observed
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Cardiovascular
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LTF/LF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LTF/LF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LTF/LF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LTF/LF. You can calculate the concentration of Mouse LTF/LF in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
62.5-4000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
37.5 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse LTF/LF concentrations in serum, plasma and other biological fluids.

UniProtKB · P08071

Lactotransferrin

TRFL_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P08071
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ltf
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
Lactoferrin
EC number
3.4.21.-
Processing
Precursor
Secondary accessions
P70690, Q61799, Q8CBA0, Q922P2
Protein keywords

PTM

Disulfide bondGlycoprotein

Molecular function

HydrolaseProteaseSerine protease

Biological process

ImmunityIon transportIron transportOsteogenesisTransport

Ligand

IronMetal-binding

Technical term

Reference proteome

Domain

RepeatSignal

Cellular component

Secreted

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