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Mouse β-EP (Beta-Endorphin) ELISA Kit

CAT: 0763-E-EL-M0184-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-M0184-01Size:24 Tests
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Abbreviation
Β-EP
UniProt
P01193
Cross Reactivity
This kit recognizes Mouse β-EP in samples.No significant cross-reactivity or interference between Mouse β-EP and analogues was observed
Target
B-EP
Type
Competitive-ELISA
Applications
ELISA
Field of Research
Cancer; Metabolism; Neuroscience; Signal transduction
Assay Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse β-EP. During the reaction, Mouse β-EP in the sample or standard competes with a fixed amount of Mouse β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse β-EP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse β-EP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Performance Time
2 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.63-1000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
9.38 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.mdpi.com/2227-9059/9/9/1185
Citation 02
https://link.springer.com/article/10.1007/s12035-018-1176-8
Citation 03
https://www.sciencedirect.com/science/article/pii/S0028390817301089
Citation 04
https://www.sciencedirect.com/science/article/pii/S0028390818307986
Citation 05
https://journals.sagepub.com/doi/abs/10.1177/1744806917754142
Citation 06
https://www.sciencedirect.com/science/article/pii/S0006291X16319532
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse β-EP concentrations in serum, plasma and other biological fluids.

UniProtKB · P01193

Pro-opiomelanocortin

COLI_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P01193
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Pomc
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
POMC
EC number
—
Processing
Precursor
Secondary accessions
P01200, Q544U4
Protein keywords

PTM

AcetylationAmidationCleavage on pair of basic residuesGlycoproteinPhosphoprotein

Molecular function

EndorphinHormone

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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