Human β-EP (Beta-Endorphin) ELISA Kit

• Abbreviation:

  Β-EP

• UniProt:

  P01189

• Cross Reactivity:

  This kit recognizes Human β-EP in samples.No significant cross-reactivity or interference between Human β-EP and analogues was observed

• Target:

  B-EP

• Type:

  Competitive-ELISA

• Applications:

  ELISA

• Field of Research:

  Cancer; Metabolism; Neuroscience; Signal transduction

• Assay Principle:

  This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human β-EP. During the reaction, Human β-EP in the sample or standard competes with a fixed amount of Human β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human β-EP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human β-EP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2 h 30 min

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  15.63-1000 pg/mL

• Precision:

  Both intra-CV and inter-CV are < 10%.

• Recovery:

  80%-120%

• Sensitivity:

  9.38 pg/mL

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  2-8°C,12 months

• Citation 01:

  https://www.sciencedirect.com/science/article/pii/S1382668922001727

• Citation 02:

  https://www.nature.com/articles/s41398-018-0263-y

• Citation 03:

  https://www.nature.com/articles/s41401-018-0202-8

• Citation 04:

  https://www.nature.com/articles/s41401-019-0241-9

• Citation 05:

  https://www.sciencedirect.com/science/article/pii/S0143417922000208

• Citation 06:

  https://www.hindawi.com/journals/mi/aa/965925/abs/

• Citation 07:

  https://www.sciencedirect.com/science/article/pii/S0091305718303691

• Citation 08:

  https://journals.viamedica.pl/endokrynologia_polska/article/view/71523

• Detection Instrument:

  Microplate reader

• Species:

  Human

• Recommended Usage:

  This ELISA kit applies to the in vitro quantitative determination of Human β-EP concentrations in serum, plasma and other biological fluids.