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Human KL (Klotho) ELISA Kit

CAT: 0763-E-EL-H5451-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-H5451-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
KL
UniProt
Q9UEF7
Cross Reactivity
This kit recognizes Human KL in samples. No significant cross-reactivity or interference between Human KL and analogues was observed
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Metabolism; Developmental biology; Signal transduction
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human KL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human KL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human KL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human KL. You can calculate the concentration of Human KL in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.31-20 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
0.19 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://ard.bmj.com/content/81/Suppl_1/1248.1.abstract
Citation 02
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403741/
Citation 03
https://pubmed.ncbi.nlm.nih.gov/35349421/
Citation 04
https://www.frontiersin.org/articles/10.3389/fphys.2022.856088/full?utm_source=dlvr.it&utm_medium=twitter
Citation 05
https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8573245/
Citation 06
https://www.karger.com/Article/Abstract/492244
Citation 07
https://link.springer.com/article/10.1007/s12032-021-01595-7
Citation 08
https://link.springer.com/article/10.1007/s40620-021-00972-9
Citation 09
https://link.springer.com/article/10.1007/s00005-023-00669-w
Citation 10
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7526019/
Citation 11
https://peerj.com/articles/12295/
Citation 12
https://link.springer.com/article/10.1186/s12882-022-02773-z
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human KL concentrations in serum, plasma and other biological fluids.

UniProtKB · Q9UEF7

Klotho

KLOT_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9UEF7
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
KL
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.2.1.31
Processing
Precursor
Secondary accessions
Q5VZ95, Q96KV5, Q96KW5, Q9UEI9, Q9Y4F0
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembraneSecreted

Disease

Disease variant

PTM

Glycoprotein

Molecular function

GlycosidaseHormoneHydrolase

Domain

RepeatSignalTransmembraneTransmembrane helix

Ligand

Vitamin D

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