Products for Research Use Only
RegisterLogin

Human PAF (Platelet Activating Factor) ELISA Kit

CAT: 0763-E-EL-H2199-01Size: 24 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0763-E-EL-H2199-01Size:24 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Abbreviation
PAF
Cross Reactivity
This kit recognizes Human PAF in samples. No significant cross-reactivity or interference between Human PAF and analogues was observed
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Epigenetics and Nuclear Signaling; Biochemicals
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PAF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PAF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PAF. You can calculate the concentration of Human PAF in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
78.13-5000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
46.88 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.mdpi.com/2072-6643/14/12/2502
Citation 02
https://www.mdpi.com/2072-6643/12/2/479
Citation 03
https://nutritionandmetabolism.biomedcentral.com/articles/10.1186/s12986-020-00528-x
Citation 04
https://www.sciencedirect.com/science/article/pii/S1472979222001226
Citation 05
https://onlinelibrary.wiley.com/doi/abs/10.1111/ced.14035
Citation 06
https://content.iospress.com/articles/tumor-biology/tub220032
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human PAF concentrations in serum, plasma and other biological fluids.

Related Products

CatalogName
E-CL-H1304-01Human PAF (Platelet Activating Factor) CLIA Kit
E-CL-M0554CLIA kit for Mouse PAF (Platelet Activating Factor)
E-CL-R0527CLIA kit for Rat PAF (Platelet Activating Factor)
E-EL-H2200ELISA kit for Human PAFAHIB3 (Platelet Activating Factor Acetylhydrolase Ib3)
E-EL-H2216ELISA kit for Human PF4 (Platelet Factor 4)
E-EL-H6184-01Human PF4 (Platelet Factor 4) ELISA Kit
E-EL-H2215ELISA kit for Human PF3 (Platelet Factor 3)
E-EL-M0926ELISA kit for Mouse PF4 (Platelet Factor 4)
E-EL-M0925ELISA kit for Mouse PF3 (Platelet Factor 3)
E-EL-M3080-01Mouse PF4 (Platelet Factor 4) ELISA Kit

Alternative Products

CatalogName
MBS288171-01Platelet-activating factor ELISA Kit
ECP7917-1Platelet Activating Factor ELISA Kit
E0526GeELISA Kit For Platelet-activating factor
AP78703-01Platelet-activating factor acetylhydrolase
E4631-100Platelet Activating Factor (PAF) ELISA Kit
MBS764342-01Porcine Platelet Activating Factor ELISA Kit
MBS735873-01Sheep Platelet activating factor ELISA Kit
MBS728249-01Rabbit Platelet activating factor ELISA Kit
MBS042247-01Hamster Platelet Activating Factor ELISA Kit
MBS026727-01Horse Platelet Activating Factor ELISA Kit

Popular Products