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Human VIM (Vimentin) ELISA Kit

CAT: 0763-E-EL-H1094-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-H1094-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
VIM
UniProt
P08670
Cross Reactivity
This kit recognizes Human VIM in samples. No significant cross-reactivity or interference between Human VIM and analogues was observed
Target
VIM; FLJ36605
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Developmental biology; Neuroscience; Signal transduction; Stem cells
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VIM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VIM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VIM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VIM. You can calculate the concentration of Human VIM in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
7.81-500 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
4.69 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.mdpi.com/984018
Citation 02
https://www.mdpi.com/1494566
Citation 03
https://www.mdpi.com/2075-4418/12/8/1939
Citation 04
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0238117
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human VIM concentrations in serum, plasma and other biological fluids.

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