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Human CPP (Copeptin) ELISA Kit

CAT: 0763-E-EL-H0851-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-H0851-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
CPP
UniProt
P01185
Cross Reactivity
This kit recognizes Human CPP in samples.No significant cross-reactivity or interference between Human CPP and analogues was observed
Target
CT-proAVP; C-Terminal Provasopressin; Vasopressin-Neurophysin 2-Copeptin
Type
Competitive-ELISA
Applications
ELISA
Field of Research
Neuroscience; Signal transduction
Assay Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human CPP. During the reaction, Human CPP in the sample or standard competes with a fixed amount of Human CPP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human CPP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human CPP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Performance Time
2 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.25-2000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
18.75 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://journals.lww.com/jhypertension/Fulltext/9900/The_relationship_between_arginine_vasopressin_gene.189.aspx
Citation 02
https://journals.lww.com/jhypertension/Abstract/2017/09002/_OP_1A_03__COPEPTIN_LEVELS_IN_PATIENTS_WITH.3.aspx
Citation 03
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0250035
Citation 04
https://www.mdpi.com/2075-4418/12/6/1435
Citation 05
https://www.mdpi.com/1251082
Citation 06
https://onlinelibrary.wiley.com/doi/abs/10.1111/jch.13374
Citation 07
https://journals.viamedica.pl/cardiology_journal/article/view/41893
Citation 08
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731524/
Citation 09
https://onlinelibrary.wiley.com/doi/abs/10.1111/ped.15355
Citation 10
https://www.sciencedirect.com/science/article/pii/S0735675720303478
Citation 11
https://www.degruyter.com/document/doi/10.1515/tjb-2020-45s208/html
Citation 12
https://clinicalhypertension.biomedcentral.com/articles/10.1186/s40885-022-00221-6
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human CPP concentrations in serum, plasma and other biological fluids.

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