Human C3a (Complement Component 3a) ELISA Kit

CAT: 0763-E-EL-H0818-01Size: 24 TestsDry Ice: NoHazardous: No
CAT#:0763-E-EL-H0818-01Size:24 Tests
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Abbreviation
C3a
UniProt
P01024
Cross Reactivity
This kit recognizes Human C3a in samples. No significant cross-reactivity or interference between Human C3a and analogues was observed
Target
C3a-desArg; ASP
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Immunology; Signal transduction
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C3a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C3a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C3a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C3a. You can calculate the concentration of Human C3a in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.31-20 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
0.19 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://europepmc.org/article/pmc/pmc9468265?javascript_support=no
Citation 02
https://www.frontiersin.org/articles/10.3389/fimmu.2020.535010/full
Citation 03
https://www.nature.com/articles/s41598-022-10936-z
Citation 04
https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0009508
Citation 05
https://www.sciencedirect.com/science/article/pii/S1381514821000869
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human C3a concentrations in serum, plasma and other biological fluids.

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