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Human PSA (Prostate Specific Antigen) ELISA Kit

CAT: 0763-E-EL-H0091-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-H0091-01Size:24 Tests
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Abbreviation
PSA
UniProt
P07288
Cross Reactivity
This kit recognizes Human PSA in samples. No significant cross-reactivity or interference between Human PSA and analogues was observed
Target
APS; KLK2A1; hK3; Kallikrein-3; Kallikrein-Related Peptidase 3; Gamma-seminoprotein
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Immunology; Neuroscience; Signal transduction; Tags and Cell Markers
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PSA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PSA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PSA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PSA. You can calculate the concentration of Human PSA in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.31-20 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
0.19 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://pubs.acs.org/doi/abs/10.1021/am505726b
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human PSA concentrations in serum, plasma and other biological fluids.

UniProtKB · P07288

Prostate-specific antigen

KLK3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P07288
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
KLK3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
PSA
EC number
3.4.21.77
Processing
Precursor
Secondary accessions
C9JXH3, G3V0H4, G3XAE3, Q15096, Q16272, Q86TG8, Q8IXI4
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

PTM

Disulfide bondGlycoproteinZymogen

Molecular function

HydrolaseProteaseSerine protease

Cellular component

Secreted

Domain

Signal

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