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TXA2 (Thromboxane A2) ELISA Kit

CAT: 0763-E-EL-0057-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-0057-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
TXA2
Cross Reactivity
This kit recognizes Universal TXA2 in samples.No significant cross-reactivity or interference between Universal TXA2 and analogues was observed
Target
TXA2
Type
Competitive-ELISA
Applications
ELISA
Field of Research
Cardiovascular; Metabolism; Signal transduction; Cancer; Epigenetics and Nuclear Signal
Assay Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal TXA2. During the reaction, Universal TXA2 in the sample or standard competes with a fixed amount of Universal TXA2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal TXA2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal TXA2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Performance Time
2 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.63-1000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
9.38 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://cmjournal.biomedcentral.com/articles/10.1186/s13020-021-00424-3
Citation 02
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868340/
Citation 03
https://www.sciencedirect.com/science/article/pii/S0002944017312300
Detection Instrument
Microplate reader
Species
Universal
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of TXA2 concentrations in serum, plasma and other biological fluids.

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