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ELISA Kit for Thromboxane A2 (TXA2)

CAT: 0338-TRV-055260-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0338-TRV-055260-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Field of Research
Signal transduction; Infection immunity; Endocrinology; Hormone metabolism
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to TXA2 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled TXA2 analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of TXA2 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of TXA2 in the sample.
Assay Performance Time
2h
Sample Type
Serum, plasma and other biological fluids
Detection Range
12.35-1,000pg/mL
Precision
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Thromboxane A2 (TXA2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Thromboxane A2 (TXA2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Sensitivity
The minimum detectable dose of this kit is typically less than 5.04pg/mL
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Format
48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity
This assay has high sensitivity and excellent specificity for detection of Thromboxane A2 (TXA2) . No significant cross-reactivity or interference between Thromboxane A2 (TXA2) and analogues was observed.
Product Name Synonym
Thromboxane A2
Method
Competitive Inhibition
Organism Species
Pan-species (General)

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