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DA (Dopamine) ELISA Kit

CAT: 0763-E-EL-0046-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-0046-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
DA
Cross Reactivity
This kit recognizes Universal DA in samples.No significant cross-reactivity or interference between Universal DA and analogues was observed
Target
2- (3;4-dihydroxyphenyl) ethylamine;3;4-dihydroxyphenethylamine;3-hydroxytyramine; DA; Intropin; Revivan; Oxytyramine
Type
Competitive-ELISA
Applications
ELISA
Field of Research
Neuroscience
Assay Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal DA. During the reaction, Universal DA in the sample or standard competes with a fixed amount of Universal DA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal DA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal DA in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Performance Time
2 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.25-2000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
18.75 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://pubmed.ncbi.nlm.nih.gov/34964929/
Citation 02
https://www.nature.com/articles/s41419-022-04562-w
Citation 03
https://www.mdpi.com/article/10.3390/antiox11071361
Citation 04
https://www.sciencedirect.com/science/article/pii/S0753332222015037
Citation 05
https://www.sciencedirect.com/science/article/pii/S0753332222008265
Citation 06
https://onlinelibrary.wiley.com/doi/abs/10.1002/mnfr.202200481
Citation 07
https://www.sciencedirect.com/science/article/pii/S0925400519315473
Citation 08
https://www.mdpi.com/1172448
Citation 09
https://www.mdpi.com/1422-0067/19/8/2241
Citation 10
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403741/
Citation 11
https://www.mdpi.com/1449146
Citation 12
https://www.nature.com/articles/s41598-022-21054-1
Detection Instrument
Microplate reader
Species
Universal
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of DA concentrations in serum, plasma and other biological fluids.

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