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DHT (Dihydrotestosterone) ELISA Kit

CAT: 0763-E-EL-0031-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-0031-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
DHT
Cross Reactivity
This kit recognizes Universal DHT in samples.No significant cross-reactivity or interference between Universal DHT and analogues was observed
Type
Competitive-ELISA
Applications
ELISA
Field of Research
Signal transduction; Developmental biology
Assay Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal DHT. During the reaction, Universal DHT in the sample or standard competes with a fixed amount of Universal DHT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal DHT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal DHT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Performance Time
2 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
39.06-2500 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
23.44 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.sciencedirect.com/science/article/pii/S0141813022007279
Citation 02
https://www.sciencedirect.com/science/article/pii/S0014299921004878
Citation 03
https://www.mdpi.com/2076-3417/12/12/6121
Detection Instrument
Microplate reader
Species
Universal
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of DHT concentrations in serum, plasma and other biological fluids.

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