Rabbit anti Mouse IgM (Fc specific)

Rabbit anti Mouse IgM (Fc specific)

• Background: The reactivity of the antiserum is restricted to the Fc part of the IgM molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal mouse serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgM. No precipitation reaction is obtained with purified IgG, IgA, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgM from serum or plasma. This antiserum is not intended for use in non-precipitating antibodybinding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
• CAS Number: 9007-83-4
• Host: Rabbit
• Clone: Polyclonal
• Source: Highly purified homogenous IgM isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Assay Principle: Precipitation assays. In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
• Form: Delipidated, heat inactivated, lyophilized, stable whole antiserum; No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of pooled; Normal rabbit serum; No foreign proteins added; Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Storage Conditions: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C; Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance of the antiserum Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day