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RecombinantFGF-basic (154aa), Human

CAT: 0013-GTR18991569-01Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18991569-01Size:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Fibroblast Growth Factor-basic (FGF-basic), also known as FGF-2, is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, bFGF has the β trefoil structure. In vivo, bFGF is produced by a variety of cells, including cardiomycotes, fibroblasts, and vascular cells. bFGF regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. bFGF can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of bFGF can produce beneficial cardioprotection during acute heart injury.Recombinant human Fibroblast Growth Factor-basic (rhFGF-basic) produced in E.coli is a single non-glycosylated polypeptide chain containing 154 amino acids. A fully biologically active molecule, rhFGF-basic has a molecular mass of 17.1 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Product Name Alternative
Fibroblast Growth Factor-basic, FGF-2, FGF2, HBGF-2, Prostatropin
Source
Escherichia coli.
Field of Research
Neuroscience
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% by SDS-PAGE analysis.
Bioactivity
ED50 4 x 10ˆ6 units/mg.
Form
Lyophilized after extensive dialysis against PBS.
Molecular Weight
17.1 kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant human Fibroblast Growth Factor-basic (rhFGF-basic) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-basic remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Notes
For research use only.
Applications Notes
Reconstituted in ddH2O at 100 μg/mL.
Preservative
Lyophilized after extensive dialysis against PBS.
AA Sequence
AAG SITTLPALPE DGGSGAFPPG HFKDPKRLYC KNGGFFLRIH PDGRVDGVRE KSDPHIKLQL QAEERGVVSI KGVCANRYLA MKEDGRLLAS KCVTDECFFF ERLESNNYNT YRSRKYTSWY VALKRTGQYK LGSKTGPGQK AILFLPMSAK S

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