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RecombinantKGF/FGF-7, Mouse

CAT: 0013-GTR18990496-01Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18990496-01Size:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Keratinocyte Growth Factor (KGF) is a highly specific epithelial mitogen produced by fibroblasts and mesenchymal stem cells. KGF belongs to the heparin binding Fibroblast Growth Factor (FGF) family, and is known as FGF-7. However, in contrast to FGF-1, which binds to all known FGF receptors with high affinity, KGF only binds to a splice variant of the FGF receptor, FGFR2-IIIb. FGFR2-IIIb is expressedby most epithelial cells, indicating KGF’s roleas a paracrine mediator. KGF induces the differentiation and proliferation of various epithelial cells such as keratinocytes in the epidermis, hair follicles and sebaceous glands., KGF is also responsible for wound repair of various tissuesincluding lung, bladder, and kidney. Recombinant mouse Keratinocyte Growth Factor (rmKGF) produced in E.coli is a single non-glycosylated polypeptide chain containing 163 amino acids. A fully biologically active molecule, rmKGF has a molecular mass of 18.7 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Product Name Alternative
FGF7, Keratinocyte Growth Factor, Fibroblast Growth Factor-7, HBGF-7, HBGF7
Source
Escherichia coli.
Field of Research
Stem Cell & Developmental Biology
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% by SDS-PAGE and HPLC analyses.
Bioactivity
ED50 5.0x 10ˆ5 units/mg.
Form
Lyophilized after extensive dialysis against PBS.
Molecular Weight
18.7 kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant mouse Keratinocyte Growth Factor (rmKGF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmKGF remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Notes
For research use only.
Applications Notes
Reconstituted in PBS at 100 μg/ml.
Preservative
Lyophilized after extensive dialysis against PBS.
AA Sequence
CND MSPEQTATSV NCSSPERHTR SYDYMEGGDI RVRRLFCRTQ WYLRIDKRGK VKGTQEMKNS YNIMEIRTVA VGIVAIKGVE SEYYLAMNKE GKLYAKKECN EDCNFKELIL ENHYNTYASA KWTHSGGEMF VALNQKGIPV KGKKTKKEQK TAHFLPMAIT

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