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RecombinantNANOG-TAT, Human

CAT: 0013-GTR18990476-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18990476-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NANOG is a transcription factor involved with self-renewal of inner cell mass and embryonic stem (ES) cells by functioning in concert with other factors such as POU5F1 (Oct-4) and SOX2. Nanog imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages, and blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes.Recombinant human NANOG-TAT (rhNANOG-TAT) produced in E.coli is a single chain, 318 amino acids non-glycosylated polypeptide. A fully biologically active molecule, rhNANOG-TAT has a molecular mass of 36.2kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Product Name Alternative
NANOG
Source
Escherichia coli.
Field of Research
Signal Transduction
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% by SDS-PAGE and HPLC analyses.
Form
Sterile Filtered solution contains 10mM PB, 300mM NaCl, pH 7.4.
Molecular Weight
36.2 kDa, analyzed by reducing SDS-PAGE.
Storage Conditions
Recombinant human NANOG-TAT (rhNANOG-TAT) remains stable up to 1-2 weeks at 4°C from date of receipt. For long term storage, aliquot and store at -20°C or -80°C. Avoid repeated freezing and thawing cycles.
Notes
For research use only.
Applications Notes
Reconstituted in ddH2O or PBS at 100 μg/ml.
Preservative
Sterile Filtered solution contains 10mM PB, 300mM NaCl, pH 7.4.
AA Sequence
MSVDPACPQ SLPCFEASDC KESSPMPVIC GPEENYPSLQ MSSAEMPHTE TVSPLPSSMD LLIQDSPDSS TSPKGKQPTS AENSVAKKED KVPVKKQKTR TVFSSTQLCV LNDRFQRQKY LSLQQMQELS NILNLSYKQV KTWFQNQRMK SKRWQKNNWP KNSNGVTQKA SAPTYPSLYS SYHQGCLVNP TGNLPMWSNQ TWNNSTWSNQ TQNIQSWSNH SWNTQTWCTQ SWNNQAWNSP FYNCGEESLQ SCMQFQPNSP ASDLEAALEA AGEGLNVIQQ TTRYFSTPQT MDLFLNYSMN MQPEDVGGYG RKKRRQRRR

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