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RecombinantTNF-α, RhesusMacaque

CAT: 0013-GTR18990452-01Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18990452-01Size:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Tumor Necrosis Factor-Alpha (TNF-α) plays a major role in regulating growth, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells. In addition to inducing hemorrhagic necrosis of tumors, studies indicate TNF is involved in certain types of tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, Crohn's disease, rheumatoid arthritis and graft-versus-host disease.Recombinant Rhesus Macaque TNF-α produced in E.coli is a single, non-glycosylated polypeptide chain containing 157 amino acids. A fully biologically active molecule, Recombinant Rhesus Macaque TNF-α has a molecular mass of 17.4 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Product Name Alternative
TNF-alpha, TNF-α; Tumornecrosis factor ligand super family member 2, TNF-a; TNFa, Cachectin, DIF, TNFA, TNFSF 2
Source
Escherichia coli.
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% as analyzed by SDS-PAGE& HPLC
Bioactivity
ED50 1.6 x 10ˆ7 units/mg.
Form
Lyophilized after extensive dialysis against PBS
Molecular Weight
17.4 kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant Rhesus Macaque TNF-α, remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Rhesus Macaque TNF-α should be stable up to 1 week at 4°C or up to 3 months at -20°C.
Notes
For research use only.
Applications Notes
Reconstituted in ddH2O at 100 μg/ml.
Preservative
Lyophilized after extensive dialysis against PBS
AA Sequence
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELTDNQLVV PSEGLYLIYS QVLFKGQGCP SNHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINLPD YLDFAESGQV YFGIIAL

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