Products for Research Use Only

Human RPE65 Protein

CAT: 0013-GTR18989156-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18989156-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This Human RPE65 Protein spans the amino acid sequence from region 2-533aa. Purity: Greater than 85% as determined by SDS-PAGE.
Product Name Alternative
(All-trans-retinyl-palmitate hydrolase) (Lutein isomerase) (Meso-zeaxanthin isomerase) (Retinal pigment epithelium-specific 65 kDa protein) (Retinol isomerase)
UniProt
Q16518
Expression Region
2-533aa
Tag
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Source
Baculovirus
Origin
Homo sapiens (Human)
Field of Research
Metabolism Research
Purity
Greater than 85% as determined by SDS-PAGE.
Form
Liquid or Lyophilized powder
Molecular Weight
64.7 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
For research use only.
Applications Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Preservative
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
AA Sequence
SIQVEHPAGGYKKLFETVEELSSPLTAHVTGRIPLWLTGSLLRCGPGLFEVGSEPFYHLFDGQALLHKFDFKEGHVTYHRRFIRTDAYVRAMTEKRIVITEFGTCAFPDPCKNIFSRFFSYFRGVEVTDNALVNVYPVGEDYYACTETNFITKINPETLETIKQVDLCNYVSVNGATAHPHIENDGTVYNIGNCFGKNFSIAYNIVKIPPLQADKEDPISKSEIVVQFPCSDRFKPSYVHSFGLTPNYIVFVETPVKINLFKFLSSWSLWGANYMDCFESNETMGVWLHIADKKRKKYLNNKYRTSPFNLFHHINTYEDNGFLIVDLCCWKGFEFVYNYLYLANLRENWEEVKKNARKAPQPEVRRYVLPLNIDKADTGKNLVTLPNTTATAILCSDETIWLEPEVLFSGPRQAFEFPQINYQKYCGKPYTYAYGLGLNHFVPDRLCKLNVKTKETWVWQEPDSYPSEPIFVSHPDALEEDDGVVLSVVVSPGAGQKPAYLLILNAKDLSEVARAEVEINIPVTFHGLFKKS

UniProtKB · Q16518

Retinoid isomerohydrolase

RPE65_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q16518
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
RPE65
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.1.1.64
Processing
—
Secondary accessions
A8K1L0, Q5T9U3
Protein keywords

PTM

AcetylationLipoproteinPalmitatePhosphoprotein

Cellular component

Cell membraneCytoplasmEndoplasmic reticulumMembraneMicrosome

Technical term

Direct protein sequencingProteomics identificationReference proteome

Disease

Disease variantLeber congenital amaurosisRetinitis pigmentosa

Molecular function

HydrolaseIsomerase

Ligand

IronMetal-binding

Biological process

Lipid metabolismSensory transductionVision